8/8/2023 0 Comments Touchdown pcr vs gradient pcrIn this manner, desired PCR products are selectively increased with little or no amplification of nonspecific targets over the course of PCR ( Figure 2).ĭNA templates containing high GC content (>65%) can be difficult to amplify because of the stronger hydrogen bonds between G and C bases. Once the annealing temperature reaches, or “touches down”, at the optimal temperature (usually 3–5☌ lower than the lowest primer T m), it is maintained throughout the remaining cycles for primer annealing. To overcome this challenge, the annealing temperature is often decreased 1☌ at every cycle of the initial few cycles to produce a sufficient yield of the desired amplicon. While preventing primer-dimers and nonspecific primer binding, the higher annealing temperatures may result in lower PCR yield due to increased dissociation of primers from their intended target. As such, higher annealing temperatures reduce nonspecific PCR products and promote specific amplification at the start of PCR (learn more about PCR annealing step). Higher temperatures help destabilize the formation of primer-dimers and nonspecific primer-template complexes, thus minimizing undesirable amplification. In touchdown PCR, the annealing temperature of the first few cycles is set to be a few degrees higher than the highest melting temperature (T m) of the primers. We will offer our best products, services and most favorable prices to guests all over the world.Another approach to promoting specificity is to modify the PCR cycling parameters. We warmly welcome our esteemed clients and friends to visit us and forward your advice and suggestions to us. LongGene can also provide OEM services for prominent companies and customize instruments to meet required specifications. By the end of 2004, LongGene brand Thermal Cyclers have reached North America, South America, Europe, Southeast Asia and South Africa. Coupled with a 2 year warranty and cheap maintenance, they will give you a peace of mind.įrom 2002, our flagship product Thermal Cycler model MG25+ began to export overseas and LongGene Company became the first manufacturer to export its own Peltier-based Thermal Cycler in China. They have been used by the Biochemistry and Cell Institute of the Chinese Academy of Science, Beijing University, Zhejiang University, and the 1st Military University, all receiving excellent praise. The world renowned products have been indispensable for teaching and research. In 2005 we also passed the ISO9001: 2000 and received the CE mark. In addition, we have developed new series products like the Dry Bath Incubator, Gel Documentation system, etc. All products adopt the most advanced PELTIER technology, importing core parts with the result that its speed, accuracy of results and reliability of system are all second to none compared to those imported. LongGene Company possesses the whole product line of Thermal Cyclers, from 16 wells to 96 wells, from STANDARD type to GRADIENT and Multi-purpose type. is a High-Tech enterprise that develops state of the art advanced life-science instruments. Hangzhou LongGene Scientific Instruments Co., Ltd. Global switch power supply: 85V ~ 265V, 50-60Hz Max. Yes,PC remote control to manage multl units across the newwork Yes, Provide precise information for the whole experiment Yes, create PCR protocols with only a few clicks Yes, an Infinite incubation time at low temperature for overnight working With password-based authentication prevents from view, copy and alteration of protocolsĪutomatically calculate the melting and annealing temperature with two primer sequences 60,000 nesting cyclesġ-120 sec, available for Long PCR & Touchdown PCR 15,000 programs onboard, unlimited storage of protocols with USB flash driveģ0 Steps, multiple nesting cycle availableġ00 Typical cycles, max. Innovative "TOP-OPEN" technology, protection from over- pressure Steplessly Adjustable Lid, accommodates all kinds of PCR tubes,strips and plates better than traditional gradient function 6-zone temperature can be set independently.
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